TDP_human_MERFISH
1. Pre-analysis Overview
1.1 Submission Form
1.2 MERFISH Data Quality
The summaries present the data quality assessment automatically generated by MERSCOPE for each experiment. We mainly focus on the transcripts level for each sample. So we’re looking for high density in transcripts, based on the transcripts count per field of view (FOV), transcript density in FOV, and frequency of transcripts detected.
Generally, log10 transcript count > 4.0 for human brain sample in most FOC can be considered as a good quality standard.
Need to note that the low accuracy in DAPI cell boundary is not a concern, as a self-designed cell segmentation processing will take over this task.
1.3 MERFISH Sample Index
| Batch | Sample | Index | Genotype | Data Path |
|---|---|---|---|---|
| 1 | H250 | Control_1 | Control | Y:/Lab/MERFISH_Imaging_data_2/202407221453_20240722Maize28H250BP1738h271x01_VMSC00101/region_0 |
| 1 | MN28 | ALS_1 | MN | Y:/Lab/MERFISH_Imaging_data_2/202407221453_20240722Maize28H250BP1738h271x01_VMSC00101/region_1 |
| 2 | H260 | Control_2 | Control | Y:/Lab/MERFISH_Imaging_data_2/202407221100_20240722105620240722Maize13H260xBP1738x2_VMSC05201/region_0 |
| 2 | MN13 | ALS_2 | MN | Y:/Lab/MERFISH_Imaging_data_2/202407221100_20240722105620240722Maize13H260xBP1738x2_VMSC05201/region_1 |
| 3 | H264 | Control_3 | Control | Y:/Lab/MERFISH_Imaging_data_2/202407261252_20240726MaizeMN32H264BP1738x03_VMSC00101/region_0 |
| 3 | MN32 | ALS_3 | MN | Y:/Lab/MERFISH_Imaging_data_2/202407261252_20240726MaizeMN32H264BP1738x03_VMSC00101/region_1 |
| 4 | H262 | Control_4 | Control | Y:/Lab/MERFISH_Imaging_data_2/202408051335_20240805MaizeMN40H262BP1738x05_VMSC00101/region_0 |
| 4 | MN40 | ALS_4 | MN | Y:/Lab/MERFISH_Imaging_data_2/202408051335_20240805MaizeMN40H262BP1738x05_VMSC00101/region_1 |